Comparison of Serum VEGF, Total Testosterone, and Fasting Insulin Levels Between Female University Students with Polycystic Ovary Syndrome and Healthy Controls: A Case-Control Study

Description

PCOS is a common metabolic glandular disorder characterised by hyperandrogenism, insulin resistance, and changes in ovarian angiogenesis. Multiple studies and a systematic review indicate elevated levels of endothelial growth factor (VEGF) in blood samples collected in EDTA tubes from patients, which are associated with increased ovarian vascular perfusion and may contribute to ovulation disorders. Insulin is associated with stimulating the production of VEGF in granulocytes, and testosterone is a primary indicator of hyperandrogenism that characterises the syndrome. This case-control study aims to compare levels of VEGF, total testosterone, and fasting insulin between female undergraduate students with PCOS and healthy students, in addition to (Capillary Blood Glucose), which is measured simultaneously with the withdrawal of the venous sample using a (glucometer). Blood samples from 10 infected women and 10 healthy women (with 10 backup samples) will be collected in the first phase, with the possibility of expanding the volume when funding is available. The indicators are measured by ELISA technology, and the samples are arranged overlappingly on the plate to minimise technical bias. The findings are expected to contribute to the understanding of the interaction between the vascular, hormonal and metabolic axis of PCOS in young women, and to provide preliminary local data that may support greater prospective research and contribute to improving knowledge about women's reproductive health