Assessment of Biological Water Quality and Contamination with Q-PCR

This project is based on implementing an innovative approach for rapid and precise water quality measure. It starts with the collection of water samples followed by direct and comprehensive extraction of total DNA from the microorganism's population present in water samples. Custom-designed primers and probes are employed to selectively target genetic markers unique to specific contaminants. Quantitative PCR (qPCR) is then used to amplify these sequences, with fluorescence-based detection systems—such as DNA-binding dyes or hydrolysis probes—providing dynamic, real-time insights into amplification progress. The intensity of fluorescence correlates directly with the original quantity of target DNA, enabling accurate assessment of contamination levels. To enhance throughput and cost-efficiency, multiplex qPCR assays are incorporated to allow the simultaneous detection of multiple microbial pathogens. This project emphasizes the powerful diagnostic capability of qPCR in quantifying both pathogenic species and biological indicators within water sources. By applying this technology, stakeholders in agriculture, environmental science and public health can achieve earlier and more precise detection of biological pollutants. Ultimately, this contributes to safeguarding public access to clean water, an essential resource facing increasing pressure not only in Palestine but also in the entire world.